Plasmid_Backbone

Part:BBa_K3789008

Designed by: MEI YING YI   Group: iGEM21_UM_Macau   (2021-09-26)


pFA6a

pFA6a is a precursor to plasmids carrying kanMX and other selector modules( HA tag, ADH1 terminator). And this plasmid is a commonly used backbone plasmid for generating epitope tagging vectors. The most common vector type is yeast genomic targeting used for saccharomyces cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1852
    Illegal SpeI site found at 1876
    Illegal PstI site found at 29
    Illegal PstI site found at 616
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1852
    Illegal SpeI site found at 1876
    Illegal PstI site found at 29
    Illegal PstI site found at 616
    Illegal NotI site found at 3
    Illegal NotI site found at 1889
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1852
    Illegal BglII site found at 406
    Illegal BamHI site found at 41
    Illegal BamHI site found at 124
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1852
    Illegal SpeI site found at 1876
    Illegal PstI site found at 29
    Illegal PstI site found at 616
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1852
    Illegal SpeI site found at 1876
    Illegal PstI site found at 29
    Illegal PstI site found at 616
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1906
    Illegal BsaI.rc site found at 3087
    Illegal SapI site found at 2004


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Categories
Parameters
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